Recent Publications

For a complete list of publications from our and collaborators' labs, please visit PubMed.

2021

Shea AE, Stocki JA, Himpsl SD, Smith SN, Mobley HLT. Loss of an Intimin-Like Protein Encoded on a Uropathogenic E. coli Pathogenicity Island Reduces Inflammation and Affects Interactions with the Urothelium. Infect Immun. 2021 Dec 6;:IAI0027521. doi: 10.1128/IAI.00275-21. [Epub ahead of print] PubMed PMID: 34871042.

Anderson MA, Brown AN, Pirani A, Smith SN, Photenhauer AL, Sun Y, Snitkin ES, Bachman MA, Mobley HLT. Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection. mBio. 2021 July 6; doi: 10.1128/mBio.01114-21 PMID: 34225485.

Mike LA, Stark AJ, Forsyth VS, Vornhagen J, Smith SN, Bachman MA, Mobley HLT. A systematic analysis of hypermucoviscosity and capsule reveals distinct and overlapping genes that impact Klebsiella pneumoniae fitness. PLoS Pathog. 2021 Mar;17(3):e1009376. doi: 10.1371/journal.ppat.1009376. eCollection 2021 Mar. PubMed PMID: 33720976; PubMed Central PMCID: PMC7993769.

Holmes CL, Anderson MT, Mobley HLT, Bachman MA. Pathogenesis of Gram-Negative Bacteremia. Clin Microbiol Rev. 2021 Jun 16;34(2). doi: 10.1128/CMR.00234-20. Print 2021 Jun 16. Review. PubMed PMID: 33692149.

2020

Frick-Cheng AE, Sintsova A, Smith SN, Krauthammer M, Eaton KA, Mobley HLT. The Gene Expression Profile of Uropathogenic Escherichia coli in Women with Uncomplicated Urinary Tract Infections Is Recapitulated in the Mouse Model. mBio. 2020 Aug 11;11(4). doi: 10.1128/mBio.01412-20. PubMed PMID: 32788379.

Jiang W, Ubhayasekera W, Breed MC, Norsworthy AN, Serr N, Mobley HLT, Pearson MM, Knight SD. MrpH, a new class of metal-binding adhesin, requires zinc to mediate biofilm formation. PLoS Pathog. 2020 Aug 11;16(8):e1008707. doi: 10.1371/journal.ppat.1008707. [Epub ahead of print] PubMed PMID: 32780778.

Johnson AO, Forsyth V, Smith SN, Learman BS, Brauer AL, White AN, Zhao L, Wu W, Mobley HLT, Armbruster CE. Transposon Insertion Site Sequencing of Providencia stuartii: Essential Genes, Fitness Factors for Catheter-Associated Urinary Tract Infection, and the Impact of Polymicrobial Infection on Fitness Requirements. mSphere. 2020 May 27;5(3). doi: 10.1128/mSphere.00412-20. PubMed PMID: 32461277.

Weakland DR, Smith SN, Bell B, Tripathi A, Mobley HLT. The Serratia marcescens siderophore, serratiochelin, is necessary for full virulence during bloodstream infection. Infect Immun. 2020 May 11;. doi: 10.1128/IAI.00117-20. [Epub ahead of print] PubMed PMID: 32393508.

Shea AE, Marzoa J, Himpsl SD, Smith SN, Zhao L, Tran L, Mobley HLT. Identification of Escherichia coli CFT073 fitness factors during urinary tract infection using an ordered transposon library. Appl Environ Microbiol. 2020 May 1;. doi: 10.1128/AEM.00691-20. [Epub ahead of print] PubMed PMID: 32358013.

Forsyth VS, Himpsl SD, Smith SN, Sarkissian CA, Mike LA, Stocki JA, Sintsova A, Alteri CJ, Mobley HLT. Optimization of an Experimental Vaccine To Prevent Escherichia coli Urinary Tract Infection. mBio. 2020 Apr 28;11(2). doi: 10.1128/mBio.00555-20. PubMed PMID: 32345645.

Himpsl SD, Shea AE, Zora J, Stocki JA, Foreman D, Alteri CJ, Mobley HLT. The oxidative fumarase FumC is a key contributor for E. coli fitness under iron-limitation and during UTI. PLoS Pathog. 2020 Feb 27;16(2):e1008382. doi: 10.1371/journal.ppat.1008382. [Epub ahead of print] PubMed PMID: 32106241.

Kitamoto S, Alteri CJ, Rodrigues M, Nagao-Kitamoto H, Sugihara K, Himpsl SD, Bazzi M, Miyoshi M, Nishioka T, Hayashi A, Morhardt TL, Kuffa P, Grasberger H, El-Zaatari M, Bishu S, Ishii C, Hirayama A, Eaton KA, Dogan B, Simpson KW, Inohara N, Mobley HLT, Kao JY, Fukuda S, Barnich N, Kamada N. Dietary L-serine confers a competitive fitness advantage to Enterobacteriaceae in the inflamed gut. Nat Microbiol. 2020 Jan;5(1):116-125. doi: 10.1038/s41564-019-0591-6. Epub 2019 Nov 4. PubMed PMID: 31686025; PubMed Central PMCID: PMC6925351.

2019

Crépin S, Ottosen EN, Chandler CE, Sintsova A, Ernst RK, Mobley HLT. The UDP-GalNAcA biosynthesis genes gna-gne2 are required to maintain cell envelope integrity and in vivo fitness in multi-drug resistant Acinetobacter baumannii. Mol Microbiol. 2020 Jan;113(1):153-172. doi: 10.1111/mmi.14407. Epub 2019 Nov 19. PubMed PMID: 31680352; PubMed Central PMCID: PMC7007346.

Sintsova A, Frick-Cheng AE, Smith S, Pirani A, Subashchandrabose S, Snitkin ES, Mobley H. Genetically diverse uropathogenic Escherichia coli adopt a common transcriptional program in patients with UTIs. Elife. 2019 Oct 21;8. doi: 10.7554/eLife.49748. PubMed PMID: 31633483; PubMed Central PMCID: PMC6802966.

Vornhagen J, Sun Y, Breen P, Forsyth V, Zhao L, Mobley HLT, Bachman MA. The Klebsiella pneumoniae citrate synthase gene, gltA, influences site specific fitness during infection. PLoS Pathog. 2019 Aug;15(8):e1008010. doi: 10.1371/journal.ppat.1008010. eCollection 2019 Aug. PubMed PMID: 31449551; PubMed Central PMCID: PMC6730947.

Anderson MT, Mitchell LA, Sintsova A, Rice KA, Mobley HLT. Sulfur Assimilation Alters Flagellar Function and Modulates the Gene Expression Landscape of Serratia marcescens. mSystems. 2019 Aug 6;4(4). doi: 10.1128/mSystems.00285-19. PubMed PMID: 31387930; PubMed Central PMCID: PMC6687942.

Sarkissian CA, Alteri CJ, Mobley HLT. UTI patients have pre-existing antigen-specific antibody titers against UTI vaccine antigens. Vaccine. 2019 Aug 14;37(35):4937-4946. doi: 10.1016/j.vaccine.2019.07.031. Epub 2019 Jul 15. PubMed PMID: 31320216; PubMed Central PMCID: PMC6812515.

Mobley HLT. Proteus mirabilis Overview. Methods Mol Biol. 2019;2021:1-4. doi: 10.1007/978-1-4939-9601-8_1. PubMed PMID: 31309490.

Pearson MM, Himpsl SD, Mobley HLT. Insertional Mutagenesis Protocol for Constructing Single or Sequential Mutations. Methods Mol Biol. 2019;2021:61-76. doi: 10.1007/978-1-4939-9601-8_7. PubMed PMID: 31309496.

Himpsl SD, Mobley HLT. Siderophore Detection Using Chrome Azurol S and Cross-Feeding Assays. Methods Mol Biol. 2019;2021:97-108. doi: 10.1007/978-1-4939-9601-8_10. PubMed PMID: 31309499.

Himpsl SD, Pearson MM, Mobley HLT. Using Hemagglutination, Surface Shearing, and Acid Treatment to Study Fimbriae in Proteus mirabilis. Methods Mol Biol. 2019;2021:109-120. doi: 10.1007/978-1-4939-9601-8_11. PubMed PMID: 31309500.

Smith SN, Himpsl SD, Mobley HLT. Vaccination to Protect Against Proteus mirabilis Challenge Utilizing the Ascending Model of Urinary Tract Infection. Methods Mol Biol. 2019;2021:201-215. doi: 10.1007/978-1-4939-9601-8_18. PubMed PMID: 31309507.

Forsyth VS, Mobley HLT, Armbruster CE. Transposon Insertion Site Sequencing in a Urinary Tract Model. Methods Mol Biol. 2019;2021:297-337. doi: 10.1007/978-1-4939-9601-8_25. PubMed PMID: 31309514.

Alteri CJ, Himpsl SD, Shea AE, Mobley HLT. Flexible Metabolism and Suppression of Latent Enzymes Are Important for Escherichia coli Adaptation to Diverse Environments within the Host. J Bacteriol. 2019 Aug 15;201(16). doi: 10.1128/JB.00181-19. Print 2019 Aug 15. PubMed PMID: 31160397; PubMed Central PMCID: PMC6657593.

Armbruster CE, Forsyth VS, Johnson AO, Smith SN, White AN, Brauer AL, Learman BS, Zhao L, Wu W, Anderson MT, Bachman MA, Mobley HLT. Twin arginine translocation, ammonia incorporation, and polyamine biosynthesis are crucial for Proteus mirabilis fitness during bloodstream infection. PLoS Pathog. 2019 Apr;15(4):e1007653. doi: 10.1371/journal.ppat.1007653. eCollection 2019 Apr. PubMed PMID: 31009518; PubMed Central PMCID: PMC6497324.

2018

Waack U, Warnock M, Yee A, Huttinger Z, Smith S, Kumar A, Deroux A, Ginsburg D, Mobley HLT, Lawrence DA, Sandkvist M. CpaA Is a Glycan-Specific Adamalysin-like Protease Secreted by Acinetobacter baumannii That Inactivates Coagulation Factor XII. mBio. 2018 Dec 18;9(6). doi: 10.1128/mBio.01606-18. PubMed PMID: 30563903; PubMed Central PMCID: PMC6299215.

Luterbach CL, Mobley HLT. Cross Talk between MarR-Like Transcription Factors Coordinates the Regulation of Motility in Uropathogenic Escherichia coli. Infect Immun. 2018;86(12):e00338-18. Published 2018 Nov 20. doi:10.1128/IAI.00338-18; PMCID: PMC6246914.

Anderson MT, Mitchell LA, Zhao L, Mobley HLT. Citrobacter freundii fitness during bloodstream infection. Sci Rep. 2018 Aug 7;8(1):11792. doi: 10.1038/s41598-018-30196-0. PubMed PMID: 30087402; PubMed Central PMCID: PMC6081441.

Debnath I, Stringer AM, Smith SN, Bae E, Mobley HLT, Wade JT, Pearson MM. MrpJ Directly Regulates Proteus mirabilis Virulence Factors, Including Fimbriae and Type VI Secretion, during Urinary Tract Infection. Infect Immun. 2018 Oct;86(10). doi: 10.1128/IAI.00388-18. Print 2018 Oct. PubMed PMID: 30082479; PubMed Central PMCID: PMC6204732.

Armbruster CE, Smith SN, Mody L, Mobley HLT. Urine Cytokine and Chemokine Levels Predict Urinary Tract Infection Severity Independent of Uropathogen, Urine Bacterial Burden, Host Genetics, and Host Age. Infect Immun. 2018 Sep;86(9). doi: 10.1128/IAI.00327-18. Print 2018 Sep. PubMed PMID: 29891542; PubMed Central PMCID: PMC6105902.

Crépin S, Ottosen EN, Peters K, Smith SN, Himpsl SD, Vollmer W, Mobley HLT. The lytic transglycosylase MltB connects membrane homeostasis and in vivo fitness of Acinetobacter baumannii. Mol Microbiol. 2018;109(6):745-762. ; PubMed Central PMCID: PMC6185781.

Luterbach CL, Forsyth VS, Engstrom MD, Mobley HLT. TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic Escherichia coli. mSphere. 2018;3(3):e00222-18. Published 2018 May 16. doi:10.1128/mSphere.00222-18. ; PubMed Central PMCID: PMC5956150.

Forsyth VS, Armbruster CE, Smith SN, Pirani A, Springman AC, Walters MS, Nielubowicz GR, Himpsl SD, Snitkin ES, Mobley HLT.. Rapid Growth of Uropathogenic Escherichia coli during Human Urinary Tract Infection. MBio. 2018 Mar 6;9(2)PubMed PMID: 29511075; PubMed Central PMCID: PMC5844997.

Sintsova A, Smith S, Subashchandrabose S, Mobley HL. Role of Ethanolamine Utilization Genes in Host Colonization during Urinary Tract Infection. Infect Immun. 2018 Mar;86(3)PubMed PMID: 29229730; PubMed Central PMCID: PMC5820945.

Dbeibo L, van Rensburg JJ, Smith SN, Fortney KR, Gangaiah D, Gao H, Marzoa J, Liu Y, Mobley HLT, Spinola SM. Evaluation of CpxRA as a Therapeutic Target for Uropathogenic Escherichia coli Infections. Infect Immun. 2018 Mar;86(3)PubMed PMID: 29311237; PubMed Central PMCID: PMC5820967.

Armbruster CE, Mobley HLT, Pearson MM. Pathogenesis of Proteus mirabilis Infection. EcoSal Plus. 2018 Feb;8(1)PubMed PMID: 29424333; NIHMSID: NIHMS925686; PubMed Central PMCID: PMC5880328.

2017

Packiriswamy N, Gandy J, Smith SN, Mobley HLT, Sordillo LM, Subashchandrabose S. Distinct Signature of Oxylipid Mediators of Inflammation during Infection and Asymptomatic Colonization by E coli in the Urinary Bladder. Mediators Inflamm. 2017;2017:4207928. PubMed PMID: 29445256; PubMed Central PMCID: PMC5763092.

Mike LA, Tripathi A, Blankenship CM, Saluk A, Schultz PJ,Tamayo-Castillo G, Sherman DH, Mobley HLT. Discovery of nicoyamycin A, an inhibitor of uropathogenic Escherichia coli growth in low iron environments. Chem Commun (Camb). 2017 Nov 28;53(95):12778-12781. PubMed PMID: 29139494; NIHMSID: NIHMS920854; PubMed Central PMCID: PMC5706644.

Alteri CJ, Himpsl SD, Zhu K, Hershey HL, Musili N, Miller JE, Mobley HLT. Subtle variation within conserved effector operon gene products contributes to T6SS-mediated killing and immunity. PLoS Pathog. 2017 Nov;13(11):e1006729. PubMed PMID: 29155899; PubMed Central PMCID: PMC5714391.

Waack U, Johnson TL, Chedid K, Xi C, Simmons LA, Mobley HLT, Sandkvist M. Targeting the Type II Secretion System: Development, Optimization, and Validation of a High-Throughput Screen for the Identification of Small Molecule Inhibitors. Front Cell Infect Microbiol. 2017;7:380. PubMed PMID: 28894700; PubMed Central PMCID: PMC5581314.

Anderson MT, Mitchell LA, Mobley HLT. Cysteine Biosynthesis Controls Serratia marcescens Phospholipase Activity. J Bacteriol. 2017 Aug 15;199(16)PubMed PMID: 28559296; PubMed Central PMCID: PMC5527384.

Armbruster CE, Forsyth-DeOrnellas V, Johnson AO, Smith SN, Zhao L, Wu W, Mobley HLT. Genome-wide transposon mutagenesis of Proteus mirabilis: Essential genes, fitness factors for catheter-associated urinary tract infection, and the impact of polymicrobial infection on fitness requirements. PLoS Pathog. 2017 Jun;13(6):e1006434. PubMed PMID: 28614382; PubMed Central PMCID: PMC5484520.

Anderson MT, Mitchell LA, Zhao L, Mobley HLT. Capsule Production and Glucose Metabolism Dictate Fitness during Serratia marcescens Bacteremia. MBio. 2017 May 23;8(3)PubMed PMID: 28536292; PubMed Central PMCID: PMC5442460.

Armbruster CE, Prenovost K, Mobley HL, Mody L. How Often Do Clinically Diagnosed Catheter-Associated Urinary Tract Infections in Nursing Homes Meet Standardized Criteria?. J Am Geriatr Soc. 2017 Feb;65(2):395-401. PubMed PMID: 27858954; NIHMSID: NIHMS802726; PubMed Central PMCID: PMC5310979.

Armbruster CE, Smith SN, Johnson AO, DeOrnellas V, Eaton KA, Yep A, Mody L, Wu W, Mobley HL . The Pathogenic Potential of Proteus mirabilis Is Enhanced by Other Uropathogens during Polymicrobial Urinary Tract Infection. Infect Immun. 2017 Feb;85(2)PubMed PMID: 27895127; PubMed Central PMCID: PMC5278182.