Publications 2018-2024

For a complete list of publications from our and collaborators' labs, please visit PubMed.

2024

Holmes CL, Albin OR, Mobley HLT, Bachman MA. Bloodstream infections: mechanisms of pathogenesis and opportunities for intervention. Nat Rev Microbiol. 2024 Oct 17. doi: 10.1038/s41579-024-01105-2. Online ahead of print. PMID: 39420097.

Shea AE, Forsyth VS, Stocki JA, Mitchell TJ, Frick-Cheng AE, Smith SN, Hardy SL, Mobley HLT. Emerging roles for ABC transporters as virulence factors in uropathogenic Escherichia coli. Proc Natl Acad Sci U S A. 2024 Apr 16;121(16):e2310693121. doi: 10.1073/pnas.2310693121. Epub 2024 Apr 12. 38607934.

Frick-Cheng AE, Shea AE, Roberts JR, Smith SN, Ohi MD, Mobley HLT. Iron limitation induces motility in uropathogenic E. coli CFT073 partially through action of LpdA. mBio. 2024 Jul 17;15(7):e0104824. doi: 10.1128/mbio.01048-24. Epub 2024 Jun 14. PMID: 38874412.

Anderson MT, Himpsl SD, Kingsley LG, Smith SN, Bachman MA, Mobley HLT. Infection characteristics among Serratia marcescens capsule lineages. bioRxiv [Preprint]. 2024 Aug 24:2024.08.23.609398. doi: 10.1101/2024.08.23.609398. PMID: 39229111.

Fitzgerald MJ, Pearson MM, Mobley HLT. Proteus mirabilis UreR coordinates cellular functions required for urease activity. J Bacteriol. 2024 Apr 18;206(4):e0003124. doi: 10.1128/jb.00031-24. Epub 2024 Mar 27. PMID: 38534115.

Fitzgerald MJ, Scavone A, Moolaveesala C, Pearson MM, Mobley HLT. Development of a suite of Proteus mirabilis-derived urea-inducible promoters. Appl Environ Microbiol. 2024 Oct 30:e0127324. doi: 10.1128/aem.01273-24. Online ahead of print. PMID: 39475285.

Stocki JA, Fleck RC, Nguyen IB, Walde R, Mobley HLT, Shea AE. Asymptomatic bacteriuria screening for developing countries using a modified water quality test kit. Appl Environ Microbiol. 2024 Oct 30:e0156724. doi: 10.1128/aem.01567-24. Online ahead of print. PMID: 39475268.

Mobley HLT, Anderson MT, Moricz BS, Severin GB, Holmes CL, Ottosen EN, Eichler T, Gupta S, Paudel S, Sinha R, Mason S, Himpsl SD, Brown AN, Gaca M, Kiser CM, Clarke TH, Fouts DE, DiRita VJ, Bachman MA. Fitness factor genes conserved within the multi-species core genome of Gram-negative Enterobacterales species contribute to bacteremia pathogenesis. PLoS Pathog. 2024 Aug 23;20(8):e1012495. doi: 10.1371/journal.ppat.1012495. eCollection 2024 Aug. PMID: 39178317.

2023

Khadka S, Ring BE, Walker RS, Krzeminski LR, Pariseau DA, Hathaway M, Mobley HLT, Mike LA. Urine-mediated suppression of Klebsiella pneumoniae mucoidy is counteracted by spontaneous Wzc variants altering capsule chain length. mSphere. 2023 Oct 24;8(5):e0028823. doi: 10.1128/msphere.00288-23. Epub 2023 Aug 23. PMID: 37610214.

Pearson MM, Shea AE, Pahil S, Smith SN, Forsyth VS, Mobley HLT. Organ agar serves as physiologically relevant alternative for in vivo bacterial colonization. Infect Immun. 2023 Nov 16;91(11):e0035523. doi: 10.1128/iai.00355-23. Epub 2023 Oct 18. PMID: 37850748.

Frick-Cheng AE, Shea AE, Roberts JR, Smith SN, Ohi MD, Mobley HLT. Altered motility in response to iron-limitation is regulated by lpdA in uropathogenic E. coli CFT073. bioRxiv [Preprint]. 2023 Sep 28:2023.09.27.559868. doi: 10.1101/2023.09.27.559868. PMID: 37808639.

Brown AN, Anderson MT, Smith SN, Bachman MA, Mobley HLT. Conserved metabolic regulator ArcA responds to oxygen availability, iron limitation, and cell envelope perturbations during bacteremia. mBio. 2023 Sep 8;14(5):e0144823. doi: 10.1128/mbio.01448-23. Epub ahead of print. PMID: 37681955.

Holmes CL, Wilcox AE, Forsyth V, Smith SN, Moricz BS, Unverdorben LV, Mason S, Wu W, Zhao L, Mobley HLT, Bachman MA. Klebsiella pneumoniae causes bacteremia using factors that mediate tissue-specific fitness and resistance to oxidative stress. PLoS Pathog. 2023 Jul 18;19(7):e1011233. doi: 10.1371/journal.ppat.1011233. PMID: 37463183

Mason S, Vornhagen J, Smith SN, Mike LA, Mobley HLT, Bachman MA. The Klebsiella pneumoniae ter Operon Enhances Stress Tolerance. Infect Immun. 2023 Feb 16;91(2):e0055922. doi: 10.1128/iai.00559-22. Epub 2023 Jan 18. PMID: 36651775; PMCID: PMC9933665.

2022

Shea AE, Frick-Cheng AE, Smith SN, Mobley HLT. Phenotypic Assessment of Clinical Escherichia coli Isolates as an Indicator for Uropathogenic Potential. mSystems. 2022 Dec 20;7(6):e0082722. doi: 10.1128/msystems.00827-22. Epub 2022 Nov 29. PubMed PMID: 36445110; PubMed Central PMCID: PMC9765037.

Pearson MM, Pahil S, Forsyth VS, Shea AE, Mobley HLT. Construction of an Ordered Transposon Library for Uropathogenic Proteus mirabilis HI4320. Microbiol Spectr. 2022 Dec 21;10(6):e0314222. doi: 10.1128/spectrum.03142-22. Epub 2022 Nov 15. PubMed PMID: 36377916; PubMed Central PMCID: PMC9769666.

Holmes CL, Smith SN, Gurczynski SJ, Severin GB, Unverdorben LV, Vornhagen J, Mobley HLT, Bachman MA. The ADP-Heptose Biosynthesis Enzyme GmhB is a Conserved Gram-Negative Bacteremia Fitness Factor. Infect Immun. 2022 Jul 21;90(7):e0022422. doi: 10.1128/iai.00224-22. Epub 2022 Jun 28. PubMed PMID: 35762751; PubMed Central PMCID: PMC9302095.

Niemiec MJ, Kapitan M, Himmel M, Döll K, Krüger T, Köllner TG, Auge I, Kage F, Alteri CJ, Mobley HLT, Monsen T, Linde S, Nietzsche S, Kniemeyer O, Brakhage AA, Jacobsen ID. Augmented Enterocyte Damage During Candida albicans and Proteus mirabilis Coinfection. Front Cell Infect Microbiol. 2022;12:866416. doi: 10.3389/fcimb.2022.866416. eCollection 2022. PubMed PMID: 35651758; PubMed Central PMCID: PMC9149288.

Frick-Cheng AE, Sintsova A, Smith SN, Pirani A, Snitkin ES, Mobley HLT. Ferric Citrate Uptake Is a Virulence Factor in Uropathogenic Escherichia coli. mBio. 2022 May 12;:e0103522. doi: 10.1128/mbio.01035-22. [Epub ahead of print] PubMed PMID: 35546538.

Brown AN, Anderson MT, Bachman MA, Mobley HLT. The ArcAB Two-Component System: Function in Metabolism, Redox Control, and Infection. Microbiol Mol Biol Rev. 2022 Jun 15;86(2):e0011021. doi: 10.1128/mmbr.00110-21. Epub 2022 Apr 20. Review. PubMed PMID: 35442087.

Anderson MT, Himpsl SD, Mitchell LA, Kingsley LG, Snider EP, Mobley HLT. Identification of distinct capsule types associated with Serratia marcescens infection isolates. PLoS Pathog. 2022 Mar;18(3):e1010423. doi: 10.1371/journal.ppat.1010423. eCollection 2022 Mar. PubMed PMID: 35353877; PubMed Central PMCID: PMC9000132.

Fortney KR, Smith SN, van Rensburg JJ, Brothwell JA, Gardner JJ, Katz BP, Ahsan N, Duerfeldt AS, Mobley HLT, Spinola SM. CpxA Phosphatase Inhibitor Activates CpxRA and Is a Potential Treatment for Uropathogenic Escherichia coli in a Murine Model of Infection. Microbiol Spectr. 2022 Apr 27;10(2):e0243021. doi: 10.1128/spectrum.02430-21. Epub 2022 Mar 17. PubMed PMID: 35297652; PubMed Central PMCID: PMC9045377.

2021

Shea AE, Stocki JA, Himpsl SD, Smith SN, Mobley HLT. Loss of an Intimin-Like Protein Encoded on a Uropathogenic E. coli Pathogenicity Island Reduces Inflammation and Affects Interactions with the Urothelium. Infect Immun. 2022 Feb 17;90(2):e0027521. doi: 10.1128/IAI.00275-21. Epub 2021 Dec 6. PubMed PMID: 34871042.

Anderson MA, Brown AN, Pirani A, Smith SN, Photenhauer AL, Sun Y, Snitkin ES, Bachman MA, Mobley HLT. Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection. mBio. 2021 July 6; doi: 10.1128/mBio.01114-21 PMID: 34225485.

Mike LA, Stark AJ, Forsyth VS, Vornhagen J, Smith SN, Bachman MA, Mobley HLT. A systematic analysis of hypermucoviscosity and capsule reveals distinct and overlapping genes that impact Klebsiella pneumoniae fitness. PLoS Pathog. 2021 Mar;17(3):e1009376. doi: 10.1371/journal.ppat.1009376. eCollection 2021 Mar. PubMed PMID: 33720976; PubMed Central PMCID: PMC7993769.

Holmes CL, Anderson MT, Mobley HLT, Bachman MA. Pathogenesis of Gram-Negative Bacteremia. Clin Microbiol Rev. 2021 Jun 16;34(2). doi: 10.1128/CMR.00234-20. Print 2021 Jun 16. Review. PubMed PMID: 33692149. 

2020

Frick-Cheng AE, Sintsova A, Smith SN, Krauthammer M, Eaton KA, Mobley HLT. The Gene Expression Profile of Uropathogenic Escherichia coli in Women with Uncomplicated Urinary Tract Infections Is Recapitulated in the Mouse Model. mBio. 2020 Aug 11;11(4). doi: 10.1128/mBio.01412-20. PubMed PMID: 32788379. 

Jiang W, Ubhayasekera W, Breed MC, Norsworthy AN, Serr N, Mobley HLT, Pearson MM, Knight SD. MrpH, a new class of metal-binding adhesin, requires zinc to mediate biofilm formation. PLoS Pathog. 2020 Aug 11;16(8):e1008707. doi: 10.1371/journal.ppat.1008707. [Epub ahead of print] PubMed PMID: 32780778. 

Johnson AO, Forsyth V, Smith SN, Learman BS, Brauer AL, White AN, Zhao L, Wu W, Mobley HLT, Armbruster CE. Transposon Insertion Site Sequencing of Providencia stuartii: Essential Genes, Fitness Factors for Catheter-Associated Urinary Tract Infection, and the Impact of Polymicrobial Infection on Fitness Requirements. mSphere. 2020 May 27;5(3). doi: 10.1128/mSphere.00412-20. PubMed PMID: 32461277. 

Weakland DR, Smith SN, Bell B, Tripathi A, Mobley HLT. The Serratia marcescens siderophore, serratiochelin, is necessary for full virulence during bloodstream infection. Infect Immun. 2020 May 11;. doi: 10.1128/IAI.00117-20. [Epub ahead of print] PubMed PMID: 32393508. 

Shea AE, Marzoa J, Himpsl SD, Smith SN, Zhao L, Tran L, Mobley HLT. Identification of Escherichia coli CFT073 fitness factors during urinary tract infection using an ordered transposon library. Appl Environ Microbiol. 2020 May 1;. doi: 10.1128/AEM.00691-20. [Epub ahead of print] PubMed PMID: 32358013. 

Forsyth VS, Himpsl SD, Smith SN, Sarkissian CA, Mike LA, Stocki JA, Sintsova A, Alteri CJ, Mobley HLT. Optimization of an Experimental Vaccine To Prevent Escherichia coli Urinary Tract Infection. mBio. 2020 Apr 28;11(2). doi: 10.1128/mBio.00555-20. PubMed PMID: 32345645. 

Himpsl SD, Shea AE, Zora J, Stocki JA, Foreman D, Alteri CJ, Mobley HLT. The oxidative fumarase FumC is a key contributor for E. coli fitness under iron-limitation and during UTI. PLoS Pathog. 2020 Feb 27;16(2):e1008382. doi: 10.1371/journal.ppat.1008382. [Epub ahead of print] PubMed PMID: 32106241. 

Kitamoto S, Alteri CJ, Rodrigues M, Nagao-Kitamoto H, Sugihara K, Himpsl SD, Bazzi M, Miyoshi M, Nishioka T, Hayashi A, Morhardt TL, Kuffa P, Grasberger H, El-Zaatari M, Bishu S, Ishii C, Hirayama A, Eaton KA, Dogan B, Simpson KW, Inohara N, Mobley HLT, Kao JY, Fukuda S, Barnich N, Kamada N. Dietary L-serine confers a competitive fitness advantage to Enterobacteriaceae in the inflamed gut. Nat Microbiol. 2020 Jan;5(1):116-125. doi: 10.1038/s41564-019-0591-6. Epub 2019 Nov 4. PubMed PMID: 31686025; PubMed Central PMCID: PMC6925351. 

2019

Crépin S, Ottosen EN, Chandler CE, Sintsova A, Ernst RK, Mobley HLT. The UDP-GalNAcA biosynthesis genes gna-gne2 are required to maintain cell envelope integrity and in vivo fitness in multi-drug resistant Acinetobacter baumannii. Mol Microbiol. 2020 Jan;113(1):153-172. doi: 10.1111/mmi.14407. Epub 2019 Nov 19. PubMed PMID: 31680352; PubMed Central PMCID: PMC7007346.  

Sintsova A, Frick-Cheng AE, Smith S, Pirani A, Subashchandrabose S, Snitkin ES, Mobley H. Genetically diverse uropathogenic Escherichia coli adopt a common transcriptional program in patients with UTIs. Elife. 2019 Oct 21;8. doi: 10.7554/eLife.49748. PubMed PMID: 31633483; PubMed Central PMCID: PMC6802966. 

Vornhagen J, Sun Y, Breen P, Forsyth V, Zhao L, Mobley HLT, Bachman MA. The Klebsiella pneumoniae citrate synthase gene, gltA, influences site specific fitness during infection. PLoS Pathog. 2019 Aug;15(8):e1008010. doi: 10.1371/journal.ppat.1008010. eCollection 2019 Aug. PubMed PMID: 31449551; PubMed Central PMCID: PMC6730947. 

Anderson MT, Mitchell LA, Sintsova A, Rice KA, Mobley HLT. Sulfur Assimilation Alters Flagellar Function and Modulates the Gene Expression Landscape of Serratia marcescens. mSystems. 2019 Aug 6;4(4). doi: 10.1128/mSystems.00285-19. PubMed PMID: 31387930; PubMed Central PMCID: PMC6687942. 

Sarkissian CA, Alteri CJ, Mobley HLT. UTI patients have pre-existing antigen-specific antibody titers against UTI vaccine antigens. Vaccine. 2019 Aug 14;37(35):4937-4946. doi: 10.1016/j.vaccine.2019.07.031. Epub 2019 Jul 15. PubMed PMID: 31320216; PubMed Central PMCID: PMC6812515. 

Mobley HLT. Proteus mirabilis Overview. Methods Mol Biol. 2019;2021:1-4. doi: 10.1007/978-1-4939-9601-8_1. PubMed PMID: 31309490. 

Pearson MM, Himpsl SD, Mobley HLT. Insertional Mutagenesis Protocol for Constructing Single or Sequential Mutations. Methods Mol Biol. 2019;2021:61-76. doi: 10.1007/978-1-4939-9601-8_7. PubMed PMID: 31309496. 

Himpsl SD, Mobley HLT. Siderophore Detection Using Chrome Azurol S and Cross-Feeding Assays. Methods Mol Biol. 2019;2021:97-108. doi: 10.1007/978-1-4939-9601-8_10. PubMed PMID: 31309499. 

Himpsl SD, Pearson MM, Mobley HLT. Using Hemagglutination, Surface Shearing, and Acid Treatment to Study Fimbriae in Proteus mirabilis. Methods Mol Biol. 2019;2021:109-120. doi: 10.1007/978-1-4939-9601-8_11. PubMed PMID: 31309500. 

Smith SN, Himpsl SD, Mobley HLT. Vaccination to Protect Against Proteus mirabilis Challenge Utilizing the Ascending Model of Urinary Tract Infection. Methods Mol Biol. 2019;2021:201-215. doi: 10.1007/978-1-4939-9601-8_18. PubMed PMID: 31309507. 

Forsyth VS, Mobley HLT, Armbruster CE. Transposon Insertion Site Sequencing in a Urinary Tract Model. Methods Mol Biol. 2019;2021:297-337. doi: 10.1007/978-1-4939-9601-8_25. PubMed PMID: 31309514. 

Alteri CJ, Himpsl SD, Shea AE, Mobley HLT. Flexible Metabolism and Suppression of Latent Enzymes Are Important for Escherichia coli Adaptation to Diverse Environments within the Host. J Bacteriol. 2019 Aug 15;201(16). doi: 10.1128/JB.00181-19. Print 2019 Aug 15. PubMed PMID: 31160397; PubMed Central PMCID: PMC6657593. 

Armbruster CE, Forsyth VS, Johnson AO, Smith SN, White AN, Brauer AL, Learman BS, Zhao L, Wu W, Anderson MT, Bachman MA, Mobley HLT. Twin arginine translocation, ammonia incorporation, and polyamine biosynthesis are crucial for Proteus mirabilis fitness during bloodstream infection. PLoS Pathog. 2019 Apr;15(4):e1007653. doi: 10.1371/journal.ppat.1007653. eCollection 2019 Apr. PubMed PMID: 31009518; PubMed Central PMCID: PMC6497324. 

2018

Waack U, Warnock M, Yee A, Huttinger Z, Smith S, Kumar A, Deroux A, Ginsburg D, Mobley HLT, Lawrence DA, Sandkvist M. CpaA Is a Glycan-Specific Adamalysin-like Protease Secreted by Acinetobacter baumannii That Inactivates Coagulation Factor XII. mBio. 2018 Dec 18;9(6). doi: 10.1128/mBio.01606-18. PubMed PMID: 30563903; PubMed Central PMCID: PMC6299215. 

Luterbach CL, Mobley HLT. Cross Talk between MarR-Like Transcription Factors Coordinates the Regulation of Motility in Uropathogenic Escherichia coli. Infect Immun. 2018;86(12):e00338-18. Published 2018 Nov 20. doi:10.1128/IAI.00338-18; PMCID: PMC6246914. 

Anderson MT, Mitchell LA, Zhao L, Mobley HLT. Citrobacter freundii fitness during bloodstream infection. Sci Rep. 2018 Aug 7;8(1):11792. doi: 10.1038/s41598-018-30196-0. PubMed PMID: 30087402; PubMed Central PMCID: PMC6081441. 

Debnath I, Stringer AM, Smith SN, Bae E, Mobley HLT, Wade JT, Pearson MM. MrpJ Directly Regulates Proteus mirabilis Virulence Factors, Including Fimbriae and Type VI Secretion, during Urinary Tract Infection. Infect Immun. 2018 Oct;86(10). doi: 10.1128/IAI.00388-18. Print 2018 Oct. PubMed PMID: 30082479; PubMed Central PMCID: PMC6204732. 

Armbruster CE, Smith SN, Mody L, Mobley HLT. Urine Cytokine and Chemokine Levels Predict Urinary Tract Infection Severity Independent of Uropathogen, Urine Bacterial Burden, Host Genetics, and Host Age. Infect Immun. 2018 Sep;86(9). doi: 10.1128/IAI.00327-18. Print 2018 Sep. PubMed PMID: 29891542; PubMed Central PMCID: PMC6105902. 

Crépin S, Ottosen EN, Peters K, Smith SN, Himpsl SD, Vollmer W, Mobley HLT. The lytic transglycosylase MltB connects membrane homeostasis and in vivo fitness of Acinetobacter baumannii. Mol Microbiol. 2018;109(6):745-762. ; PubMed Central PMCID: PMC6185781. 

Luterbach CL, Forsyth VS, Engstrom MD, Mobley HLT. TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic Escherichia coli. mSphere. 2018;3(3):e00222-18. Published 2018 May 16. doi:10.1128/mSphere.00222-18. ; PubMed Central PMCID:  PMC5956150. 

Forsyth VS, Armbruster CE, Smith SN, Pirani A, Springman AC, Walters MS, Nielubowicz GR, Himpsl SD, Snitkin ES, Mobley HLT.. Rapid Growth of Uropathogenic Escherichia coli during Human Urinary Tract Infection. MBio. 2018 Mar 6;9(2)PubMed PMID: 29511075; PubMed Central PMCID: PMC5844997.

Sintsova A, Smith S, Subashchandrabose S, Mobley HL. Role of Ethanolamine Utilization Genes in Host Colonization during Urinary Tract Infection. Infect Immun. 2018 Mar;86(3)PubMed PMID: 29229730; PubMed Central PMCID: PMC5820945.

Dbeibo L, van Rensburg JJ, Smith SN, Fortney KR, Gangaiah D, Gao H, Marzoa J, Liu Y, Mobley HLT, Spinola SM. Evaluation of CpxRA as a Therapeutic Target for Uropathogenic Escherichia coli Infections. Infect Immun. 2018 Mar;86(3)PubMed PMID: 29311237; PubMed Central PMCID: PMC5820967.

Armbruster CE, Mobley HLT, Pearson MM. Pathogenesis of Proteus mirabilis Infection. EcoSal Plus. 2018 Feb;8(1)PubMed PMID: 29424333; NIHMSID: NIHMS925686; PubMed Central PMCID: PMC5880328.